Screening of ATRA downstream genes in hepatocellular carcinoma cells by expression profile microarray
Abstract：Objective To carry out expression profile microarray analysis after hepatoma carcinoma cell line-HepG2 cell are treated by all-trans retinoic acid ( ATRA ) in order to explore its effects on gene expression profile of hepatoma carcinoma cells. Methods The HepG2 cells were treated by 80μM ATRA ( trial group) and equal volume of ethanol ( control group) for 24h,then the total mRNA was extracted from the treated cells. The cDNA microarray technique was used to detect and compare the differentially expressed genes between the two groups. Results After HepG2 cells were treated by ATRA,a total 661 differentially expressed genes were screened out, moreover these genes were closely correlated to cell signal transduction, cell proliferation and cell differentiation. Conclusion The cDNA microarray technique is used to screen successfully the differentially expressed genes after HepG2 cells are treated by ATRA, which establishes the foundation for exploring further the action mechanism of ATRA on hepatocellular carcinoma, and provides also an important theoretical basis for the clinical application of ATRA to induce differentiation of liver cancer cells.
Keywords：all-trans retinoic acidhepatocellular carcinomadifferentiationgene chips
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2572-2574 )