The significance of Notch3 in nasopharyngeal carcinoma
Abstract：Objective To explore the possibility of Notch 3 as a molecular biological index to evaluate the recurrence , metastasis and prognosis of nasopharyngeal cancer .Methods Western Blot was used to detect the effects of silencing Notch 3 in nasopharyngeal carcinoma cell lines-CNE2 by small interfering RNA ( siRNA) .The sphere forming assay was performed to analyze the influence of silencing Notch 3 in CNE2 on the number of cancer stem cells .The expression levels of E-cadherin and VImentin were detected by Western Blot to observe the influence of silencing Notch 3 in CNE2 on EMT.The effects of silencing Notch3 in CNE2 on glycolysis were observed by detecting the concentration of lactic acid .The expression levels of Notch 3 were determine after CNE2 cells were treated by cisplatin in order to evaluate whether Noth 3 could be regarded as an index of chemotherapy sensitivity.Results The results by Western Blot showed that the siRNA could effectively silence the expressions of Notch3 in CNE2 cells.The results by sphere forming assay showed that the sphericity structure was obviously increased after silencing Notch3 in CNE2 cells, which suggested that silencing Notch 3 increased the number of cancer stem cells .After silencing Notch3 in CNE2 cells the expression levels of epithelial marker -E-cadherin were decreased , however , the expression levels of mesenchymal cell marker molecule -Vimentin were increased , which indicated that silencing Notch 3 promoted EMT transformation of CNE2 cells.Moreover lactate concentration was increased in CNE 2 cells after silencing Notch3,which suggested that silencing Notch 3 in CNE2 cells could increase glycolysis .Western Blot results showed that the expression levels of Notch3 were decreased after CNE2 cells were interfered by cisplatin,which indicated that Notch3 could be regarded as an index to evaluate the efficiency of chemotherapeutics .Conclusion In nasopharyngeal carcinoma cell lines-CNE2 cells, silencing Notch3 can increase the number of cancer stem cell , promote EMT transition and enhance glycolysis . The expression levels of Notch3 are decreased after CNE2 cells are interfered by cisplatin.Thus Notch3 can be regarded as an index to evaluate the recurrence , metastasis and prognosis of nasopharyngeal cancer .
Keywords：nasopharyngeal cancerNotch3cancer stem cellEMTglycolysis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2262-2266 )