The shunting action of DNA quantitative cytology analysis in HPV-gegative atypical squamous ce1ls of undetermined significance

doi:10.3969/j.issn.1002-7386.2016.14.006

Abstract：Objective To investigate the shunting action of DNA quantitative cytology analysis in HPV-negative atypical squamous ce1ls of undetermined significance (ASCUS).Methods A total of 80 patients with HPV-negative ASCUS received DNA ploid analysis of cervical cells ,then the abnormal DNA ploid was followed by cervical biopsy under colposcope . According to DNA index ( DI) ,the examination results by DNA quantitative cytology analysis were divided into 3 groups:DI<2.5, DI>2.5,DI>5,moreover, according to patient’s age,these patients were also divided into 3 groups:age≤30 years,31~49 years, age≥50 years.The abnormal detection rates of CIN and abnormal DNA quantitive examination results were analyzed and compared among the groups .Results The positive rate of abnormal DNA ploid was 20%in HPV-negative group ,and the detection rate of CIN of abnormal DNA ploid in patients with ASCUS was 38.8%.There were significant differences in the pathological examination results among different DI groups ( P <0.01).The detection rates of CIN2 and CIN3 in DI>5 group were significantly higer than those in DI >2.5 group and DI <2.5 group ( P <0.05).However there were no significant differences in the pathological examination results between DI >2.5 group and DI <2.5 group ( P >0.05).The detection rate of CIN of DNA quantitative cytology abnormality in age≥50 years group was higher than that in the other two groups,especially,the detection rate of CIN2 was 45.5%.There were significant differences in DNA quantitive examination results among different age groups ( P <0.01).Conclusion The DNA ploid analysis in patients with HPV-negative ASCUS is helpful to detect CIN ,which is an effective method for shunting action screening .Taking DI>5 as the criterion of cervical biopsy can enhance the detaction rate of CIN 2 or higher grade CIN .