A experimental stud y on the effects of mgan esiumc hlro ide on ultrastructure renovation after scai tic nerve injury in rats

doi:10.3969/j.issn.1002-7386.2016.14.001

Abstract：Objective To investigate the effects of magnesium chloride on ultrastructure renovation after sciatic nerve injury in rats .Method s Ninety SD rats were randomly divided into 3 groups: magnesium chloride group ( MgCl2 group ) , nerve growth factor group (NGF group), 0.9%sodium chloride group (NaCl group), with 30 rats in each group.Firstly,the animal models with sciatic nerve injury were established , and the injury was bridged with silicone tube to set up a nerve regeneration chamber (NRC),then 1mmol/L MgCl2,NCF,0.9% sodium chloride solution was infused into the chamber , respectively.After operation the rats were sacrificed on 4,8,12 weeks,respectively .The regenerating tissues in NRC were taken out to be fixed ,finally, the regenerating nerve fibers number and section area of regenerating nerve fibers were observed under light microscope ,moreover, the state of proliferation and development of Schwann cells in NRC and ultrastructure of medulla sheath was observed by electron microscopy .Results The regenerating nerve fibers number and section area of regenerating nerve fibers in MgCl2 group and NGF group on 4,8,12 weeks were significantly higher than those in Nacl group (P<0.05).However there were no significant differences between MgCl 2 group and NGF group ( P >0.05).In MgCl2 group and NGF group ,the regenerating nerve fibers number was increased ,the proliferation of Schwann cells was obvious and cell's shape was nearly normal ,the thickness of medulla sheath was uniformly increased .in contrast to that, in NaCl group, nerve fibers regeneration was not obvious , medulla sheath structures were blurred and twisted , the proliferation of Schwann cells was not obvious , with immature phenotype .Conclusion Magnesium chloride can promote the ultrastructure renovation after sciatic nerve injury in rats .