The regulation effects of AT1-R-JAK/STAT signal pathway on expressions of visfatin in hypertrophy myocardial cells ;in vitro

doi:10.3969/j.issn.1002-7386.2016.09.003

Abstract：Objective To observe the expressions of visfatin in angiotensin Ⅱ( AngⅡ)-induced cardiomyocyte hypertrophy in vitro after blocked by different signal channel blockers,and to explore the significance of changes of visfatin levels.Methods The primary generation cardiomyocytes from Sprague-Dawley (SD) neonate rats (2~3d age) were cultured in vitro with routine medium,however, which were cultured in medium without serum after 48 hours.The cardiomyocytes were divided into different groups after 24 hours,and the intervention effects of AngⅡ were blocked by different signal channel blockers.The expression levels of visfatin in cardiomyocytes were detected by RT-PCR,Western-Blot,respectively,moreover, the expression levels of brain natriuretic peptide (BNP) in cardiomyocytes were detected by Western-Blot.Results The activity of cardiomyocytes (optical density value,OD) and expression levels of BNP were gradually increased when AngⅡconcentration at 10 -8 ,10 -7 ,10 -6 mol/L.The activity of cardiomyocytes and expression levels of BNP were decreased when AngⅡconcentration at 10 -5 mol/L.The levels of visfatin and visfatin mRNA were increased when AngⅡ concentration at 10 -8 ,10-7 ,10-6 mol/L,which reached peak at 10 -5 mol/L.There were significant differences in the activity of cardiomyocytes and expression levels of visfatin mRNA and protein when AngⅡconcentration at 10 -6 mol/L between experimental group and control group ( P <0.05).The OD value of cardiomyocytes,expression levels of visfatin mRNA and protein,expression levels of BNP protein were increased, with the time went on.The expression levels of visfatin mRNA and protein in PD123319 intervention group and AngⅡintervention group were significantly high than those in control group and telmisartan intervention group ( P <0.05).There were significant differences in the expression levels of visfatin mRNA and protein between AngⅡgroup and control group and between AG490 +AngⅡ group, SP600125 +AngⅡgroup and U0126 +AngⅡgroup ( P <0.05), moreover,which in AG490+AngⅡ group,SP600125+AngⅡ group and U0126 +AngⅡ group were significantly higher than those in control group ( P <0.05).Conclusion The enhancement of visfatin expression levels in AngⅡ-induced cardiomyocyte hypertrophy is mediated by AT1-R-JAK/STAT signal pathway.