The is gn ificanc e of Thl/Th2/Thl7/Treg in distinguishing asthma for m pneum onia in children

doi:10.3969/j.issn.1002-7386.2016.09.001

Abstract：Objective To investigate the levels of Th1 ( IFN-γ+) , regulatory T cells ( CD2+5 Foxp3 +) , helper T cells 17 (Th17), and cytokines-IL-2, IL-4, IL-17, IL-21 in peripheral blood of children with asthma and pneumonia,and to explore their significance in the pathogenesis of asthma and the differential diagnosis from pneumonia.Methods Thirty-two children with asthma ( asthma group ) and thirtys-even children with pneumonia ( pneumonia group ) were enrolled in the study,moreover, the other twenty-two healthy children were served as control group.The peripheral blood mononuclear cells ( PBMC) were separated, then the percentages of CD4+IFN-γ+,CD4+CD+25 Foxp3 +,CD4+Th17 +in PMBC were detected by flow cytometry.The expression levels of IL 2-,IL-4,IL-17 and IL-21 in plasma were detected by enzyme-linked mimunosorbent assay (ELISA).R esults The ratio[(2.09 ±1.16)%] of CD4+CD2+5Foxp3 +cells/CD4+T cell in asthma group was obviously lower than that [(5.13 ±2.41)%]in pneumonia group and that [(5.67 ±2.79)%] in control group.The cell percentage of CD4+IFN-γ+in asthma group [(8.47 ±3.23)%] was obviously lower than that in pneumonia group [(15.48 ±3.00)%] and that in control group[(14.84 ±3.74 )%].The percentage [(7.40 ±2.69)%] of CD4+Th 17 +in asthma group was significantly higher than that [(2.86 ±1.62)%] in pneumonia group and that [(2.56 ±1.18)%] in control group (P <0.05).The levels of IL-2 in asthma group and pneumonia group were significantly lower than those in control group ( P <0.05).However there were no significant differences in the levels of IL-2 between asthma group and pneumonia group ( P >0.05).The concentrations of IL-4, IL-17 and IL-21 in asthma group were significantly lower than those in pneumonia group and those in control group ( P <0.05).Conclusion The functional disorder of Th1/T2h /Th17/Treg may be involved in the pathogenesis of asthma in children,which can be used as the indexes to evaluate pathogenetic condition of bronchial asthma in children and can distinguish it from pneumonia.