The investigation of correlation between MTHFR gene polymorphism and congenital heart disease by means of GeXP ;multiplex analysis technique
Abstract：[Abstarct] Objecti ev To investigate the correlation between methylenetetrahydrofolate reductase ( MTHFR)gene polymorphism and congenital heart disease (CHD)by means of Genomelab genetic analysis system (GeXP).Methods The peripheral blood specimens of 261 children patients with CHD who received cardiac surgical procedures in Department of Cardiac Surgery of Hebei Provincial Children Hospital from February 2013 to December 2014 were collected,moreover, the peripheral blood specimens of 49 healthy children were collected as control group.The whole blood DNA was extracted from the blood specimens, then the two sensitive loci ( rs1801131 and rs1801133 ) in MTHFR gene were detected by GeXP multiplex analysis technique.The correlation between MTHFR gene polymorphism and congenital heart disease was analyzed and statistically tested by chi-square test (χ2 ) with SPSS 16.0 software.Results The GeXP gene sequencing technique could simultaneously detect four kinds of genotypes of two single nucleotide polymorphism ( SNP) .There were no significant differences in MTHFR677C/T allele and genotype frequency between CHD group and control group (χ2 =0.853, P =0.356;χ2=0.898, P =0.343).Conclusion GeXP multiplex analysis platform is a kind of multiplex analysis technique in screening susceptibility genes of CHD,with fine specificity and simple operation .The experimental results show that there are no significant differences in MTHFR allele or its genotype distribution between patients with CHD and healthy children,which can be used to explore susceptibility genes of CHD and establish the basis of methodology for large sample study later.
Keywords：congenital heart diseaseMTHFRchildren
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1143-1146 )