The effects and action mechanisms of wogonin on the proliferation, apoptosis and invasion of human laryngeal carcinoma cells (Hep-2) in vitro
Abstract：Objective To investigate the effect and potential action mechanisms of wogonin on the proliferation , apoptosis and invasion in human laryngeal carcinoma cells ( Hep-2 ) in vitro.Methods After treated with different concentrations of wogonin , the effects of wogonin on proliferation activity of Hep-2 cells were detected by CCK-8, and its effects on cell apoptosis of Hep-2 were evaluated by flow cytometry .The effects of wogonin on nucleus morphologic change of Hep-2 were measured by Hoechst staining ,and its effects on invasive ability of Hep-2 cells were detected by Transwell assay. Furthermore, the expression levels of hTERT ,Bcl-2,Bax proteins were detected by Western Blot .Results The results by CCK-8 showed that wogonin could obviously inhibit proliferation of Hep-2, with a concentration -dependent manner .The results by flow cytometry indicated that wogonin could induce apoptosis of Hep-2.Hoechst staining suggested that wogonin could results in nucleus morphologic change of Hep-2, and Transwell assay showed that wogonin could inhibit the invasive ability of Hep-2.Moreover,Western Blot indicated that wogonin could obviously decrease the expression levels of hTERT and Bcl-2 and increase the expression levels Bax ( P <0.05).Conclusion Wogonin can significantly inhibit cell proliferation , induced cell apoptosis and inhibit invasive ability of Hep-2 in vitro, and its action mechanism may be correlated to the regulation of expression levels of hTERT ,Bcl-2 and Bax protein .
Keywords：wogoninlaryngeal carcinomaHep-2 cellsanti-tumor effects
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 987-990 )