The protective effect and action mechanism of Salvia miltiorrhiza monomer-IH764-3 on aristolochci acid induced renal tubular epithelial cell injury
Abstract：Objective To investigate the protective effects and action mechanism of Salvia miltiorrhiza monomer -IH764-3 on aristolochic acid(AA) induced renal tubular epithelial cell injury .Mtehods The human renal tubular epithelial cell line-HK-C cells were cultured in DMEM medium with 10%fetal calf serum for 72 hours,which were divided into three large groups ( eight small groups ):control group , the culture solution without AA;AA group , AA was added into culture solution with final concentration of 20mg/L;observation group,AA and IH764-3 (final concentration of 20mg/L)and different concentrations of Salvia miltiorrhiza monomer-IH764-3.After cultured for 24h, 48h, 72h, the expression levels of transforming growth factor 1 (TGF-β1),tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), plasminogen activator inhibitor type 1 (PAI-1), matrix metalloproteinase 2 ( MMP-2)in culture medium were detected by enzyme-linked immunosorbent assay ( ELISA) The activity of HK-C cells was measured by MTT assay,and the expression levels of Caspase-3 mRNA were detected by Real-time quantitative PCR.Results After AA stimulation, the expression levels of TGF-β1, TIMP-1,PAI-1 were increased, however, the expression levels of MMP-2 were obviously decreased ( P <0.05).After intervention by Salvia miltiorrhiza monomer -IH764-3, the expression levels of TGF-β1, TIMP-1 and PAI-1 were decreased, however, the expression levels of MMP-2 were increased, in which the group with IH764-3 80mg/L had the best effects ( P <0.05).As compared with that in control group ,the activity of HK-C cells in AA group and observation group was decreased , however , cell apoptosis rates were increased ,moreover, the expression levels of Caspase-3 mRNA were significantly increased ( P <0.05).As compared with that in AA group , the activity of HK-C cells in observation group was obviously increased ,however, cell apoptosis rates were decreased , furthermore, the expression levels of Caspase-3 mRNA were significantly decreased ( P <0.05),with the best effects in IH764-3 80mg/L group ( P <0.05).Conclusion Salvia miltiorrhiza monomer-IH764-3 can obviously enhance the ability of HK-C cells against AA that results in cell damage ,thus,which can protect HK-C cells from injury, moreover, its action mechanism may be related with decreasing the expression levels of TGF-β1, TIMP-1, PAI-1 Caspase-3 and increasing the expressions of MMP-2.
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Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 982-986 )