Study on the expression of nucleostemin in renal cell carcinoma
Abstract：Objective To investigate the expression of nucleostemin ( NS) in renal carcinoma tissues .Methods Fifty patients with renal cell carcinoma who were treated in our hospital from May 2013 to October 2014 were enrolled in the study.All the patients were pathologically confirmed ,including 27 females and 23 females (age range:26~80 yr), the renal carcinoma specimens resected surgically were colleted and pathologically graded into G 1, G2, G3 according to Thoenes , which were clinically staged into stageⅠ, Ⅱ, Ⅲ, Ⅳ according to Robson .The expression levels of NS mRNA were detected by reverse transcription-polymerase chain reaction ( RT-PCR ) , and the correlation and significance were analyzed .Besides 10 healthy subjects were served as control group .Results The absorbance in normal renal tissues was significantly higher than that in renal carcinoma tissues ( P <0.05).However there was no significant difference in absorbance among clear cell carcinoma, chromophilic cell carcinoma, chromophobe cell carcinoma ( P >0.05).The positive expression rates of NS in renal carcinoma tissues were 45.45%,77.27%,88.23%, respectively in G1, G2, G3, there were significant differences among them ( P <0.05).The positive expression rates of NS in stageⅠ,Ⅱ,Ⅲ,Ⅳrenal carcinoma tissues were 57.14%, 61.11%,100%,100%,respectively, there were significant differences among them ( P <0.05).Moreover the positive expression rates of NS in Ⅲ, Ⅳ stages renal carcinoma tissues were significantly high than those in stageⅠ,stage Ⅱ renal carcinoma tissues,besides, the positive expression rates of NS were not correlated to patient ’s age and sex,tumor’s size and lymph node metastasis ( P >0.05).Conclusion The expression levels of NS in renal carcinoma tissues are not correlated to types of renal cell carcinoma,patient’s age and sex,tumor’s size and lymph node metastasis,however,which are related with pathological grading and clinical staging of renal carcinoma .
Keywords：renal carcinomanucleosteminpathological characteristics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 976-978 )