The action mechanism of 5-fluorouracil in treatment of colon cancer by activating TGF-β1/Smad pathway
YANG Donghai
Abstract：Objective To explore the action mechanism of 5-fluorouracil ( 5-Fu ) in treatment of colon cancer by activating TGF-β1/Smad pathway .Methods A total of 24 male Wistar rats were randomly divided into control group , azoxymethane treatment group ( AOM group ) , AOM plus 5-Fu treatment group ( AOM +5-Fu group ) , with 8 rats in each group.The expression levels of transforming growth factor-β1 (TGF-β1),TGF-βtypeⅡreceptor (TGF-βRⅡ) and Smad4 of Smad gene family members were detected by Real-time quantitative PCR , Western Blot and immunohistochemistry , respectively.Results As compared with those in control group , the expression levels of TGF-β1 mRNA,TGF-βR Ⅱ mRNA and Smad4 mRNA were significantly decreased ( P <0.01).As compared with those in AOM group, the expression levels of TGF-β1, TGF-βRⅡand Smad4 were significantly increased ( P <0.01) after 5-Fu treatment.Conclusion The 5-Fu can increase the expression levels of TGF-β1, TGF-βRⅡand Smad4 in colon cancer tissues to activate TGF-β1/Smad pathway.
Keywords：colon cancer5-fluorouraciltransforming growth factor-β1receptortransforming growth factor-βtypeⅡSmad4 protein
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 805-808 )