Effects of Lipocalin-2 silencing gene on the sensitivity of human lung cancer A549 cells to cisplatin

doi:10.3969/j.issn.1002-7386.2016.03.005

Abstract：Objective To observe the effects of Lipocalin-2 silencing gene on the sensitivity of human lung cancer A549 cells to cisplatin through silencing the expression of Lipocalin-2 gene by means of small interfering RNA( siRNA). Methods The expression levels of Lipocalin-2 protein in lung cancer tissues and 3 kinds of human lung cancer cell lines including A549,NCI-H661,NCI-H446 were detected by immunohistochemistry( SP)and Western Blot,respectively. Specific Lipocalin-2 siRNA was established and transfected into A549 cells via lentivirus,then the transfection efficiency was detected by Real-time PCR and Western Blot. Cell survival rate and inhibitory concentration 50(IC50)were measured by MTT assay. The cell apoptosis rates after treatment by cisplatin were detected by flow cytometry. Results The overexpressions of Lipocalin-2 protein were found in lung cancer tissue and the three kinds of cancer cell lines( P < 0. 05). The expression levels of Lipocalin-2 mRNA and protein were significantly decreased in A549 cells after transfection by siRNA-Lipocalin-2,at the same time,the cell survival rate was obviously decreased after induction by cisplatin,however,cell apoptosis rate was significantly increased( P < 0. 05). Conclusion The specific siRNA can effectively inhibit the expression levels of Lipocalin-2 mRNA and protein in A549 cell and can enhance the sensitivity of A549 cells to cisplatin.