Effects of oxymatrine combined with lamivudine on TLR9 signal pathway in patients with chronic hepatitis B

WANG Hao; GAO Shulin; YANG Jingjing

doi:10.3969/j.issn.1002-7386.2016.02.007

Abstract：Objective To investigate the effects of oxymatrine combined with lamivudine on TLR9 signal pathway in patients with chronic hepatitis B(CHB)in order to explore possible action mechanism of oxymatrine in treatment of CHB. Methods One hundred and twenty patients with CHB in our hospital were randomly divided into control group and observation group,with 60 patients in each group. The patients in control group,on the routine therapy,were given lamivudine, however,the patients in observation group ,on the basis of control group,were treated by oxymatrine,with a treatment course of 6 months for both groups. The therapeutic effects were observed and compared between two groups. Moreover the expression levels of TLR9,MyD88,TRAF6,IRF7 mRNA and protein in peripheral blood mononuclear cell(PBMCs)were detected by Real time-PCR and Western Blot,respectively,and the serum levels of IFN-α,IFN-γ,TNF-α were detected by enzyme linked immunosorbent assay(ELISA). Results The total effective rates in observation group and control group were 96. 67% and 70. 0% ,respectively,and there was a significant difference between two groups( P < 0. 05). As compared with those before treatment,the expression levels of TLR9,MyD88,TRAF6,IRF7 mRNA and proteins,the serum levels of IFN-α,IFN-γ, TNF-αwere significantly increased after treatment in both groups( P < 0. 05),furthermore,the increase degree in observation group was more significant than that in control group( P < 0. 05). Conclusion Oxymatrine combined with lamivudine is effective for CHB,with an obvious anti-HBV role,and its action mechanism may be related to activating TLR9 signal pathway and inducing the release of antiviral cytokines.