To study on the abnormal expression of RNA binding protein-QKI-5 in colorectal cancer tissues
Abstract：Objective To investigate the differences of expression of RNA-binding protein of QKI-5 between colorectal cancer tissues and normal rectal tissues. Methods The fresh surgical ablation specimens of colorectal carcinoma were collected,and the expression levels of QKI-5 were detected and compared by immunoblotting and Real-time PCR methods. The expression levels of QKI-5 in different differentiation levels of colorectal carcinoma tissues were observed by using the same method. The correlation between clinicopathological characteristics of colorectal cancer and QKI-5 was statistically analyzed. Results Western Blot results showed that the expression levels of QKI-5 in colorectal cancer tissues were significantly lower than those in normal rectal tissues( P < 0. 05),meanwhile,Real-time PCR also got similar results (P < 0. 05). With the continous decrease of differentiation levels of colorectal cancer tissue,the expression levels of QKI-5 were correspondingly decreased( r = - 0. 917,P < 0. 05). Furthermore QKI-5 was closely correlated to TNM staging,lymph node metastasis,distant metastasis and tumor marker - CEA( P < 0. 05). Conclusion QKI-5 may play an important role of anti - oncogene in the pathogenesis and development of colorectal carcinoma,thus,the researches taking QKI-5 as target point are of important significance for the construction of molecular biological markers in early diagnosis and research & development of biotherapeutic targets of colorectal carcinoma.
Keywords：colorectal carcinomaQKI-5 geneimmunohistochemistry
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 181-183,184 )