Inhibitory effects of alpha-TIF siRNA-loaded PLGA-TPGS nanoparticles on herpesviruses 1
YANG Chengming
WANG Kebin
Abstract：Objective To investigate the inhibitory effects of alpha-TIF siRNA-loaded PLGA-TPGS nanoparticles on herpesviruses 1(HSV1). Methods The novel biodegradable PLGA-TPGS nanoparticles(NPs)were prepared as a delivery system of small interfering ribonucleic acid(siRNA)targetingα-TIF for inhibiting HSV1,and its characteristics including size,zeta potentials,entrapment rate and in vitro release rate were represented. The cytotoxicity of α-TIF siRNA-loaded PLGA-TPGS nanoparticles on epithelial cells and HeLa cells was examined by MTT assay. The inhibitory effect of thenanoparticleson HSV1 was detected by barren spot assay. Results The size,zeta potential of PLGA-TPGS/ α-TIF-siRNA NPs was(257 ± 2. 94)nm and(31. 25 ± 1. 70)mV,respectively. The entrapment rate of siRNA was(56. 23 ± 3. 68)% ,and the in vitro release of NPs displayed biphase kinetics,that is,which reached 50% on 96 hours,then,it was slowly released. The cytotoxicity of PLGA-TPGS/ α-TIF-siRNA NPs on primary keratinocytes or HeLa cells was not observed by using MTT assay. The release of SiRNA located in NPs was observed by using fluorescent microscope. PLGA-TPGS/ α-TIF-siRNA NPs could inhibit HSV1 replication in HeLa cells. Conclusion PLGA-TPGS nanoparticles can be used as siRNA carrier. PLGA-TPGS/ α-TIF-siRNA NPs can inhibit HSV1 replication in vitro,which may become candidate drugs for HSV1 infection- related diseases including keratitis.
Keywords：herpesviruses 1alpha-gene trans-inducing factornanoparticlessiRNA
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 165-169 )