Effects of hydrogen sulfide on oxidative stress of brain tissues in rats with focal cerebral ischemia
Abstract：Objective To observe the effects of hydrogen sulfide(H2S)on oxidative stress of brain tissues in rats with focal cerebral ischemia,and to explore possible action mechanism. Methods Forty healthy male SD rats were randomly divided into five groups:sham operation group(group S),ischemia model group(group I),low-dose H2 S group(group L), median-dose H2 S group(group M)and high-dose H2 S group(group H). The rats were anesthetized by 10% chloral hydrate at a concentration of 350mg/ kg. The animal models with middle cerebral artery occlusion were established by thread ligation method. However the rats in group S underwent a surgical operation,without thread ligation. The rats in group L,M,H were given H2 S(0. 7mg,1. 4mg and 2. 8mg/ kg,respectively)at 3h after cerebral ischemia,however,the rats in group S and group I were given equal volume of 0. 9% sodium chloride solution. These rats were sacrificed at 24h after cerebral ischemia. The content of malondialdehyde(MDA),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in brain tissue were detected,furthermore,the ultrastructure changes of neurons were observed by transmission electron microscopy. Results As compared with those in group S ,the activities of SOD and GSH-PX were significantly decreased, however,the content of MDA was obviously increased in group I( P ﹤ 0. 01). The results by transmission electron microscopy showed neuron edema,mitochondria swelling,that the cristae of mitochondria was disrupted,dissolved or disappeared,the amount of organelles was obviously decreased in group I. As compared with those in group I,the activities of SOD and GSH-PX were significantly increased,however,the content of MDA was obviously decreased in group M and group H( P ﹤ 0. 05). The results by transmission electron microscopy showed slight neuron,part mitochondria swelling,that the cristae of mitochondria was partly disrupted,dissolved or disappeared,the amount of organelles was increased,moreover,the injury degree of cerebral ischemia was obviously alleviated in group M and group H. Conclusion H2 S can relieve focal cerebral ischemic injury in rats,and its action mechanism may be correlated to increasing the antioxidative stress ability of rats.
Keywords：hydrogen sulfidecerebral ischemiaoxidative stressrats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )