Construction and identification of hTfR lentivirus expression vector
Abstract：Objective To clone human transferrin receptor (hTfR) into lentivirus expression vector-pLENTI6.3 and identify the reconstructed plasmid, which will provide experimental basis for in vivo stem cell magnetic resonance ( MR) molecular imaging .Methods The hTfR gene cDNA was amplified by polymerase chain reaction ( PCR ) and cloned into pLENTI6.3 vector.The reconstructed pLENTI6.3-hTfR-IRES-EGFP plasmid was identified by colony PCR ,Bamh1 enzyme digestion and DAN sequence analysis.Results The hTfR gene fragment was constructed into pLENTI6.3 vector.The reconstructed plasmid was identified by colony PCR,enzyme digestion and DAN sequence analysis,which showed that hTfR gene sequence was exactly the same as that in Genbank.Conclusion The pLENTI6.3-hTfR-IRES-EGFP lentivirus expression vector is successfully constructed,which provides experimental basis for experimental researches about in vivo stem cell MR molecular imaging.
Keywords：hTfRlentivirus vectorstem cellmagnetic resonance imaging
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3685-3687,3688 )