Protce tive effect of the alpha-lipoic acid on oix datvi e stress in podocytes of rats with diabetic nephropathy

doi:10.3969/j.issn.1002-7386.2015.23.001

Abstract：[Abts ract] Objective To investigate the protective effect of alpha-lipoic acid ( ALA ) on oxidative stress in the podocytes of rats with diabetic nephropathy.Methods After the rat models with diabetic nephropathy were successfully established,the 10 rats were randomly selected as diabetic nephropathy group (group B), the other 10 rats were regarded as ALA intervention group (group C),at the sime time, 10 normal rats were served as control group (group A).The animals were sacrificed at th end of 13weeks ,and the renal tissues specimens were harvested .The expression levels of nephrin and podocin were detected by Western Blot and immunohistochemistry.The contents of malondialdehyde ( MDA) in renal tissues were measured by thiobarbituric acid (TAB) method and the activities of total superoxide dismutase (T-SOD), glutathione peroxidase ( GSH-PX) in renal tissues were detected by xanthine oxidase and dithio-2 nitrobenzoic acid method,respectively. Resu lts As compared with those in group A,the concentrations of MDA in group B were significantly increased, however, the acticities of T-SOD ,GSH-PX and the expression levels of nephrin, podocin were significantly decreased ( P <0.05).As compared with those in group B, the concentrations of MDA were obviously decereased, but the acticities of TS-OD ,GSH-PX and the expression levels of nephrin, podocin were significantly increased in group C ( P <0.05).Conclusion ALA can inhibit the apoptosis of podocyte, reduce the urine protein and delay the pathogenesis and development of diabetic nephropathy through antioxidative stress effects.