Clinical significance of immunological indexes and serum cytokines in patients with autoimmune diseases

SONG Aihua; WANG Xinsheng; LI Yanjing

doi:10.3969/j.issn.1002-7386.2015.22.005

Abstract：Objective To observe the changes of CD4+CD2+5 FoxP3+regulatory T cells ( Treg) , T lymphocyte cells subsets, immunoglobulin, complements, inflammatory cytokines, complements and adhesion molecules in patients with autoimmune diseases in order to explore their roles in pathogenesis and development of diseases.Methods A total of 150 patients with autoimmune diseases who were admitted into our hospital from January 2012 to January 2015 were enrolled in the study.These patients were divided into active period group ( n =80) and stable period group ( n =70) according to the active status of diseases,besides,150 healthy subjects were served as control group.The CD4+ CD2+5 FoxP3 +Treg, T lymphocyte cells subsets ( CD3+,CD4+, CD8+) and NK cells were detected by flow cytometry.Immunoglobulins ( IgA, IgG, IgM) and complements (C3, C4) were measured by turbidimetric method with automatic biochemical analyzer.Inflammatory cytokines (TNF-α, IL-10, IL-17, IFN-γ) and adhesion molecules (ICAM-1,VCAM-1,E-selectin) were detected by ELISA. Results As compared with those in control group and stable period group, CD4+CD2+5 FoxP3+Treg, CD4+, CD4+/CD8+, NK cells, C3 and C4 in active period group were significantly decreased,however, CD8+, IgA, IgG, IgM, TNF-α, IL-10, IL-17, IFN-γ, ICAM-1, VCAM-1 and E-selectin in active period group were significantly increased ( P <0.05) .Conclusion There exists inflammatory reaction and immunological function disorder in patients with autoimmune diseases,therefor the detection of immune indexes, inflammatory cytokines and adhesion molecules is helpfor to assess the patient’s immune state of and disease’s progress so as to provide experimental basis for clinical treatment.