Effect of sodium tanshinone ⅡA sulfonate combined with atorvastatin on TLR4 inflammatory signal pathway and immune function of patients with coronary heart diseases

doi:10.3969/j.issn.1002-7386.2015.22.001

Abstract：Objective To investigate the effect of sodium tanshinone ⅡA sulfonate combined with atorvastatin on TLR4 inflammatory signal pathway and immune function of patients with coronary heart diseases ( CHD) in order to explore the possible action mechanism of sodium tanshinone ⅡA sulfonate in treatment of CHD.Methods One hundred and sixty patients with coronary artery diseases who were admitted into our hospital from January 2014 to January 2015 were randomly divided into observation group ( n =80 ) and control group ( n =80 ) .The patients in control group were treated with atorvastatin,on the basis of symptomatic treatment,however, the other patients in observation group, on the basis of the control group, were treated with sodium tanshinoneⅡA sulfonate,with a treatment course of 30 days for both groups.The expression levels of TLR4 mRNA in peripheral blood were detected by Real time-PCR.The serum levels of IL-6, hs-CRP, TNF-α, MMP-9 and ox-LDL were measured by ELISA.The lymphocyte subsets ( CD3+,CD4+,CD8+, CD4+/CD8+) were detected by flow cytometry.The immunoglobulins( IgA, IgG, IgM) and complement levels ( C3, C4 ) were examined by turbidimetric inhibition immuno assay for both groups.Results As compared with those before treatment,the levels of TLR 4 mRNA,IL-6, hs-CRP,TNF-α,MMP-9,ox-LDL,IgA,IgG,IgM,C3,C4 were significantly decreased after treatment for both groups ( P <0.05),however, the levels of CD3+, CD4+, CD8+, CD4+/CD8+ were significantly increased ( P <0.05), moreover, the observation group was superior to control group in the improvement degree of these indexes ( P <0.05).Conclusion Sodium tanshinoneⅡA sulfonate combined with atorvastatin can significantly inhibit the activation of TLR4 inflammatory signal transduction pathway and can improve immunological function disorders,which may be one of the action mechanisms of sodium tanshinoneⅡA sulfonate in treatment of CHD.