Clinical significance and correlation between gene polymorphism of GP6 and deep venous thrombosis of lower limbs

LV Bonan; ZHAO Wei; SHI Xiaoming

doi:10.3969/j.issn.1002-7386.2015.21.008

Abstract：Objective To investigate the correlation between single nucleotide polymorphism (SNPs) in rs1654419 locus of GP6 gene and lower limbs deep venous thrombosis ( DVT ) in Chinese Han population around Shijiazhuang city. Methods The genotype and allele frequencies of SNPs in rs1654419 locus of GP6 gene in downtown and periphery area of Shijiazhuang city were detected by DNA direct sequencing technology in 243 cases of lower limbs DVT ( case group) and 445 cases of healthy subjects ( control group) , at the same time, the body mass index ( BMI) , plasma levels of D-Dimer and Fbg were detected for both groups.Results The BMI, plasma levels of D-Dimer and Fbg in case group were significantly higher than those in control group ( P <0.05).The gene frequencies of AA, AG and GG genotypes in case group were 16.9%, 27.6%, 55.5%,respectively,however,which in control group were 9.4%,20.9%, 69.7%,respectively.The A allele frequency (0.307) in case group was significantly higher than that (0.199) in control group ( P <0.05).According to different genotype of GG, AG, AA, the levels of Fbg and D-Dimer in both groups were increased in order ( P <0.05 ) . Logistic regression analysis results showed that AA genotype and A allele were the independent risk factor of lower limbs DVT, with OR (95%CI) being 2.951 (1.198~4.687) ( P <0.05).Conclusion The SNPs in rs1654419 locus of GP6 gene are correlated to thrombosis in patients with lower limbs DVT of Chinese Han population in downtown and periphery area of Shijiazhuang city.