Expressions of COX-2, VEGF, MMP-9 in pancreatic cancer tissue and the effects of celecoxib on expression of VEGF and MMP-9 in human pancreatic cancer cell line BXPC-3
SU Chunyong
HUO Huazhi
YANG Xiaoguang
Abstract：Objective To investigate the expressions of cyclooxygenase-2 ( COX-2 ) , vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) in pancreatic cancer tissue and the effects of celecoxib on expression of VEGF and MMP-9 in human pancreatic cancer cell line BXPC-3.Methods The expression levels of COX-2, VEGF, MMP-9 proteins were detected by immunohistochemistry ( SP) in 50 cases of primary pancreatic cancer tissue and adjacent normal tissues,moreover, the correlation between the expressions of COX-2, VEGF, MMP-9 and clinicopathological characteristics of pancreatic cancer was analyzed.The effects of celecoxib on expressions of VEGF and MMP-9 mRNA and proteins in human pancreatic cancer cell line BXPC-3 were detected by RT-QPCR and Western Blot.Results The expression levels of COX-2, VEGF, MMP-9 in pancreatic cancer tissue were significantly higher than those in adjacent normal tissue ( P <0.05 ) .The expressions of COX-2, VEGF, MMP-9 were closely correlated to proliferative activity, tumor metastasis and clinical stage of pancreatic carcinoma ( P <0.05), however,which were not related with patient’ s age and gender, anatomical site and histological grade of pancreatic carcinoma,furthermore, the expression of COX-2 was positively correlated to that of VEGF or MMP-9 ( P <0.05).With the increase of celecoxib dosage ,the expression levels of VEGF, MMP-9 mRNA and protein in pancreatic cancer cell line BXPC-3were significantly decreased , as compared with those in control group ( P <0.05 ) . Conclusion The high expression of COX-2, VEGF, MMP-9 in pancreatic carcinoma may play an important role in pathogenesis and progression of pancreatic carcinoma.Celecoxib may develop antitumor effects through down-regulating the expressions of COX-2, MMP-9 and VEGF.
Keywords：pancreatic cancercyclooxygease 2vascular endothelial growth factormatrix metalloproteinase 9celecoxib
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3205-3208 )