St udy on the role of COX-2mRNA in the process of inflammatory lesion of bone andjoint
LI Guan
GAN Lijiao
ZHONG Miaorong
Abstract：Objective To observe the changes of cyclooxygenase-2 gene ( COX-2 mRNA ) in the process of inflammatory lesion of bone and joint, and to explore its effects on the lesions.Methods Fifty -one patients with knee osteoarthritis ( case group) and 20 patients with non knee osteoarthritis ( control group) who were admitted into Department of Rheumatism Immunization Diseases of our hospital from 2010 to 2014 were enrolled in the study.According to the process of inflammatory lesion of bone and joint, these patients were redivided into early period group ( n =17) , middle period group (n=19) and late period group ( n =15).The expression levels of COX-2mRNA in fibroblast like synovial cells were detected by Real-time fluorescence quantitative PCR( RT-PCR) ,and the expression levels of COX protein were detected by Western Blot.Results The expression gray values of COX-2 mRNA and protein in fibroblast like synovial cells in case group were 0.76 ±0.11,0.85 ±0.09,respectively, both of them were significantly higher than those in control group ( P <0.05). There were significant differences in the expression gray values of COX-2mRNA and protein among early period group,middle period group,late period group ( P <0.05).The expression gray values of COX-2mRNA and protein in early period group were 0.92 ±0.09,0.98 ±0.09,respectively, both of them were significantly higher than those in middle period group,late period group and control group ( P<0.05),however, there were no significant differences in the expression gray values of COX-2mRNA and protein between middle period group and late period group ( P >0.05 ) .The expression gray values of COX-2mRNA and protein in middle period group and late period group were significantly higher than those of control group (P<0.05).Conclusio n The expression gray values of COX-2mRNA and protein in fibroblast like synovial cells in patients with knee osteoarthritis are significantly higher than those of patients with non knee osteoarthritis,moreover, the expression levels are the highest at early period,which suggests that cyclooxygenase-2 gene may play an important role in early period of inflammatory lesions of bone and joint in patients with knee osteoarthritis.
Keywords：cyclooxygenase-2 geneosteoarthritislesionsrole
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3064-3066 )