The anti-tumor effects of curcumin on human bladder cancer cell line (T24) and its effects on cell autophagy in vitro
QIU Wei
SHEN Yongqing
NI Xiaochen
Abstract：Objective To investigate the anti-tumor effects of curcumin on human bladder cancer cell line (T24) and its effects on cell autophagy in vitro.Methods The inhibition effects of curcumin on cell proliferation of T24 cell line were detected by CCK-8 assay.The cell apoptosis rate was determined by Annexin V/PI assay.The expression levels of LC3B and P62 protein were detected by Western Blot.The changes of autophagic vacuoles were observed by acridine orange staining. Results Curcumin could obviously inhibit the proliferation of T24 cells and induce cell apoptosis ( P <0.01).Curcumin induced autophagy of T24 cells, up-regulated expression levels of LC3 protein, down-regulated expression levels of P62 protein and increased the numbers of autophagic vacuoles ( P <0.01 ) .Moreover curcumin enhanced the apoptosis effect of cis-Dichlorodiamineplatinum (CDDP, P <0.01).Conclusion Curcumin can obviously inhibit the proliferation of T24 cells and induce cell apoptosis through cell autophagy, moreover, curcumin can enhance the anti-tumor effects of CDDP.
Keywords：curcuminbladder cancerapoptosisproliferationautophagy
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3054-3057 )