Effects of miRNA-34c on migration and invasion in human glioma cell line U251 and U87 in vitro
Abstract：Objective To investigate the effects of miRNA-34c on migration and invasion in human glioma cell line U251 and U87 in vitro.Methods After miR-34c was introduced into human glioma cell line U251 and U87 with liposome,the transfection efficiency was identified by quantitative PCR, and the changes of cell migration and invasion abilities were detected by Transwell chamber.Results In U87 and U251 cell lines, the expression levels of miRNA-34c-3p and miRNA-34c-5p were significantly lower than those in normal glial cells line HEB.However the expression levels of miRNA-34c-3p after transfection were significantly higher than those in normal glial cells and NC cells ( P <0.05),so were miRNA-34c-5p. After miR-34c-3p or miR-34c-5p was transfected into U251 cells or U87 cells by means of Transwell chamber, the cell numbers which migrated into the lower chamber were significantly lower than those in normal cell group and NC group. Conclusion The up-regulation of expression of miRNA-34c in glioma through transfection can inhibit the migration and invasion abilities of glioma,which provides a reliable basis for targeted therapy of brain glioma.
Keywords：gliomamiR-34c-3pmiR-34c-5pmigrationinvasion
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2888-2891 )