Effect of Her-2/neu siRNA on COX-2 expression and biological behavior of human pancreatic cancer BXPC-3 cells

HUO Huazhi; SU Yongchun; YANG Xiaoguang

doi:10.3969/j.issn.1002-7386.2015.17.005

Abstract：Objective To investigate the specific effect of silencing of Her-2/neu gene by small interfering RNA ( siRNA) on COX-2 expression and the cell biological behaviors of human pancreatic cancer cell line BXPC-3 in order to find an new therapeutic method for pancreatic cancer.Methods The expression levels of Her-2/neu, COX-2 mRNA and proteins were detected by RT-QPCR and Western Blot in human pancreatic cancer cell lines BXPC3 and normal pancreas cell line HPDE6C7.The specific Her-2/neu siRNA was synthesized and transfected into BXPC3 cells by lentivirus, and the expression levels of Her-2/neu and COX-2 were detected.The CCK-8 assay was used to analyze the proliferation of BXPC3 cells.Flow cytometry was used to determine the apoptosis of BXPC3 cells.The cell invasion was evaluated by transwell assay.Results The overexpressions of Her-2/neu, COX-2 mRNA and proteins were observed in human pancreatic cancer cell lines BXPC3. However the expressions of Her-2/neu, COX-2 mRNA and proteins were almost not detected in normal pancreas cell line HPDE6C7, there were significant differences between the two kinds of cells ( P <0.05).The Her-2/neu siRNA could remarkedly inhibit the expressions of COX-2 mRNA and proteins.The proliferation and invasiveness of BXPC3 cells in Her-2/neu siRNA group were decreased obviously.However the apoptosis of BXPC3 cells in Her-2n/eu siRNA group was significantly increased (P <0.05).Conclusion The overexpressions of Her-2/neu and COX-2 are observed in the human pancreatic cancer cell lines BXPC3,moreover, Her-2/neu gene silence can obviously inhibit proliferative and invasive abilities of the cells, furthermore, which can induce apoptosis of BXPC3 cells.