Effects of Trollius flavonoids on the expression of Trefoil factor 3 in human papillary thyroid cancer K1 cells in vitro
Abstract：Objective To investigate the inhibition effect of Trollius flavonoids on the proliferation of Trefoil factor 3 (TFF3) in human papillary thyroid cancer K1 cells in vitro, and to explore its effect on the expression of TFF3 in K1 cells.Methods The K1 cells cultured in vitro were treated by different concentrations of Trollius flavonoids for 24h,48h,72 h,respectively ,then the inhibition rate on K 1 cells growth was dectected by MTT .The changes of expression of TFF 3 protein/mRNA were detected by Western blotting and Real-time PCR, respectively.Results MTT detection showed that Trollius flavonoids had inhibition effects on proliferation and growth of K 1 cells in a dose and time dependent manner ( P <0.05). The examination results by Western blotting and Real-time PCR showed that the expression levels of TFF 3 protein and mRNA of K1 cells were decreased with the increase of flavonoids concentration ( P <0 .05 ) .Conclusion Trollius flavonoids have the inhibition effect on human throid papillary cancer cells and can inhibit the expression of TFF 3.
Keywords：Trollius flavonoidspapillary thyroid cancerTrefoil factor 3
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2405-2407 )