Effects of cholecystokinin 8 on NF-κB activity in rats with acute traumatic brain injury
Abstract：Objective To investigate the effect of cholecystokinin-8( CCK-8)on the changes of Necular Factor Kappa B(NF-κB)activity in rats with acute traumatic brain injury. Methods Sixty adult Sprague-Dawley rats weighted 250g ~ 300g were randomly divided into three groups:control group,trauma group and CCK-8 treatment group. The NF-κB activity was detected by electrophoretic mobility shift assay(EMSA)and the expression of NF-κB within cells was detected by immunohistochemistry,also the water content in brain tissue of injury part was measured by dry-wet weight method. Results Theactivity of NF-κB and water content in brain tissue in trauma group were significantly increased in different time points,as compared with those in control group,however,the NF-κB activity was obviously inhibited,moreover,the brain edema was alleviated at some extent in CCK-8 treatment group. Conclusion The activity of NF-κB in brain tissue is obviously increased immediately after acute traumatic brain injury,however,CCK-8 can effectively relieve the damage caused by secondary inflammatory reaction by inhibiting the activity of NF-κB.
Keywords：NF-κBbrain injuryCCK-8inflammation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1933-1935 )