Correlation between Mir-2 1 and hypovaria

doi:10.3969/j.issn.1002-7386.2015.10.001

Abstract：Objective To compare the differences of plasma Mir-21 expression and granular cell apoptosis between patients with hypovaria and healthy people,and to explore the influence of Mir-21 on ovarian function in order to provide theoretical basis for pathogenesis of ovarian function decline.Methods According to basic FSH,the subjects were divided into hypovaria group (FSH >10 U /L,n =20)and control group (FSH < 8 U /L,n =23).According to the database and previous study,the expression levels of Mir-21 were detected by Real-time quantitative PCR,and the apoptosis rate of granulosa cells was compared between two groups.Results The expression levels of Mir-21 in hypovaria group were significantly lower than those in control group (P <0.05),however,the apoptosis rate of granular cell was significantly higher than that of control group (P <0.05 ).The hoechst 33258 staining showed that the granulosa cells in hypovaria group were brightly stained,with karyopyknosis and irregular granular apoptotic body in cell nucleus.Conclusion The expression levels of Mir-21 in peripheral blood of patients with hypovaria are down-regulated,as compared with those in healthy people,which may result in the increase of granular cell apoptosis and abnormal development of oocyte,so as to result in hypovaria.