Effect of Liangxuejiedu prescription on the levels of CD4+CD2+5 regulatory T cells in peripheral blood of patients with primary immune thrombocytopenia

doi:10.3969/j.issn.1002-7386.2015.07.004

Abstract：Objective To observe the change of CD 4+CD2+5 regulatory T cells levels in peripheral blood of patients with primary immune thrombocytopenia ( ITP) before and after treatment with Liangxuejiedu prescription ,and to explore the effect of Liangxuejiedu prescription on the levels of CD 4+CD2+5 regulatory T cells in patients with ITP .Methods Fifty-six patients with ITP,including 12 males and 44 females,mean age 29 (age range :18~62yr),were enrolled in the study.The patients were treated with Liangxuejiedu prescription ( Dihuangzhixue capsule 2.0g once,3 times a day per os plus Shwngban decoction,one dose per day).The levels of CD4+CD2+5regulatory T cells in peripheral blood of patients before and 90 days after treatment were detected by flow cytometry .Besides 20 healthy subjects were served as controls .Results After 90-day treatment,the total effective rate was 73.2%.The levels of CD4+CD2+5 regulatory T cells in peripheral blood of patients with ITP were significantly lower than those in control group [(1.01+0.67)%vs (2.81+0.52)%, P <0.05],moreover,the levels of CD4+CD2+5 regulatory T cells in peripheral blood of patients with severe ITP were significantly lower than those of patients with non-severe ITP [(0.71 +0.23)% vs (1.48 +0.64)%, P <0.01].After 90-day treatment,the levels of CD4+CD2+5 regulatory T cells in peripheral blood of patients with ITP were significantly higher than those before treatment [(1.93 +0.53)%vs (1.01+0.67)%, P <0.05],furthermore,the levels of CD4+CD2+5 regulatory T cells in effectiveness group were significantly higher than those in inefficiency group ( P <0.05).Conclusion The levels of CD4+CD2+5 regulatory T cells in peripheral blood of patients with ITP are significantly lower than those in control group ,and the increase of levels of CD 4+CD2+5 regulatory T cells caused by Liangxuejiedu prescription may be the action mechanism in treatment of ITP .