Expression and significance of Wnt3a and Lgr5 in bladder cancer

doi:10.3969/j.issn.1002-7386.2015.06.007

Abstract：ObjectiveToinvestigatetheexpressionandsignificanceofWnt3aandLgr5inbladdercancer.Methods The expression levels of Wnt3a protein and Lgr5 protein wee detected by immunohistochemistry in 11 cases of normal tissues and 39 cases of bladder cancer tissues.The expression levels of Wnt3a mRNA and Lgr5 mRNA were detected by RT-PCR in 9 cases of normal bladder tissues and 17 cases of bladder cancer tissues.Results The results by immunohistochemistry showed the positive expression rates of Wnt3a protein were 84.6%,33.3% in bladder cancer tissues and normal bladder tissues, respectively,there were significant differences between the two kinds of tissues ( P <0.05).The positive expression rates of Lgr5 protein were 84.6%,33.3% in bladder cancer tissues and normal bladder tissues,respectively,there were significant differences between the two kinds of tissues ( P <0.05).The expression of Wnt3a was positively correlated to that of Lgr5 in bladder cancer tissues ( r =0.677, P <0.01).The results by RT-PCR showed that there was a significant difference in the ratio of optical density of Wnt3a between bladder cancer tissues and normal bladder tissues ( P <0.05),there was a significant difference in the ratio of optical density of Lgr5 between bladder cancer tissues and normal bladder tissues ( P <0.05).Conclusion The expression levels of the protein and mRNA of Wnt3a and Lgr5 in bladder cancer tissues are much higher than those in normal bladder tissues,which suggest that Wnt3a and Lgr5 may be closely related with the pathogenesis and development of bladder cancer.