Effect of ceftriaxone on chronic neuropathic pain and the expression of GLT-1 in r ats

doi:10.3969/j.issn.1002-7386.2015.04.004

Abstract：Objective To investigate the effects of ( Ceftriaxone ,Cef) on chronic neuropathic pain and the expression of GLT-1 in rats.Methods Ninety male Spague-Dawley rats were randomly divided into sham-operation group, CCI 14d group,Cef prevention group,Cef therapy group,at the same time,the two groups with intraperitoneal injection with NS were designed as control groups for the last two groups .The thermal withdrawal latency was measured in different time points , in addition,CCI 1d,CCI 4d and CCI 7d groups were designed to observe dynamic change of GLT-1 expression after CCI.The changes of GLT-1 expression in dorsal horn of spinal cord were detected by immunohistochemistry .Results The thermal hyperalgesia in rats was induced by chronic crushing injury (CCI),however,intraperitoneal injection with NS had no effect on thermal hyperalgesia induced by CCI .The thermal withdrawal latency was obviously prolonged in Cef prevention group on the 5th day,the 7th day after CCI( P <0.05),moreover,which in Cef therapy group was significantly prolonged on the 11th day, 14th day after CCI( P <0.05).The results by immunohistochemisty showed that Cef prevention group and Cef therapy group inhibited and reversed the down-regulation of GLT-1 expression at CCI later period .Conclusion The Cef can prevent and treat chronic neuropathic pain by up-regulating the expression of GLT-1.