Effects of Astragalin polysaccharides on immune function in vivo

doi:10.3969/j.issn.1002-7386.2015.04.001

Abstract：Objective To investigate the effects of Astragalin polysaccharides on immune function of mice in order to provide theoretical basis for clinical application .Methods Forty mice were randomly divided into four groups:low-dose Astragalin polysaccharide group ,median-dose Astragalin polysaccharide group ,high-dose Astragalin polysaccharide group and normal control (NC) group,with 10 mice in each group.The mice in Astragalin polysaccharide groups were given Astragalin polysaccharides 0.2ml by gavage (5mg/ml,10mg/ml,20mg/ml,respectively) per day,however,the mice in NC group were given 0.9%sodium chloride solution 0.2ml by gavage per day .After10 days, the mice were sacrificed without pain and the specimens were collected from them .The immune function parameters of the mice were examined as follows: ( 1 ) Innate immune:macrophage phagocytosis was detected; ( 2 ) Cellular immune: delayed hypersensitivity intensity was tested; ( 3 ) Humoral immune:hemolysin content in serum was examined by hemagglutination test;(4)Immune organ:spleen and thymus indexes were calculated by weighting .Results The phagocyte rate and phagocyte indexes in the three Astragalin polysaccharide groups were significantly higher than those in NC group ( P <00.1 ) .As compared with that in NC group ,the weight difference between left and right postpedes in Astragalin polysaccharide groups was significantly improved ( P <0.01).The antibody titre in Astragalin polysaccharide groups was significantly higher than that in NC group ( P <0.05).As compared with those in NC group , the spleen and thymus indexes in Astragalin polysaccharide groups were significantly increased ( P <0.01).Conclusion Astragalin polysaccharides can obviously enhance immune function of organism including innate immune ,humoural immune and cellular immune .