Experimental study on the pathophysiological change and mechanism of acute lung injury in rats with severe acute pancreatitis

doi:10.3969/j.issn.1002-7386.2015.03.007

Abstract：Objective To observe the pathophysiological change of acute lung injury in rats with severe acute pancreatitis(SAP),and to explore its pathogenesis mechanism .Methods The 240 healthy male SD(Sprague-Dawley) rats from January 2008 to 2009 January were enrolled in the study , these rats were randomly divided into 8 groups according to random digits table,7 observation groups and control group ,the observation groups included 7 different time points of SAP ,ie. 1h group,3h group,6h group,9h group,12h group,18h group,24h group and control group ,with 30 rats in each group.The changes of serum amylase , the ratio of lung tissue wet/dry weight , lung myeloperoxidase ( MPO ) activity and the lungs permeability index were detected and compared among the 8 groups.Results The lung injury score,the ratio of lung tissue wet/dry weight and lung MPO levels in observation groups were gradually increased ,as compared with those in control group , moreover,which in 7 time points were significantly higher than those in control group ( P <0.05).The lung permeability index in observation groups was gradually decreased ,as compared with that in control group ( P <0.05).The MPO levels in lung tissue were positively correlated to lung injury score ( r =0.946, P <0.01),and the ratio of lung tissue wet/dry weight was positively correlated to lung injury score ( r =0.879, P <0.01),however,lungs permeability index was negatively correlated to lung injury score( r =0.859, P <00.1 ).Conclu sion The lung injury degree of rats with SAP is gradually increased with prolongation of course of disease ,moreover ,the MPO levels in lung tissue are continually increased .