Study on protective effects and its imechanism of luteolin on acute lung injury induced by lipopolysaccharide in mice
DAI Li
DUAN Limei
YAO Zhiqing
Abstract：Objective To investigate the effect of luteolin on acute lung injury ( ALI) induced by lipopolysaccharide (LPS) in mice,and to explore possible action mechanism .Methods Ninety-six mice were randomly divided into 6 groups:notmal control group ,model group ,luteolin low-dose group luteolin median-dose group luteolin high-dose group ,dexamethasone control group ,with 8 mice in each group .The animal models with ALI were established by injecting LPS into airway of mice , then the changes of lung airway inspiratory resistance (Ri),expiratory resistance (Re) and dynamic lung compliance (Cdyn) were detected by pulmonary function test apparatus .The levels of IL-1β,IL-6,TNF-αin bronchoalveolar lavage fluid ( BALF) were determined by ELISA .The pathological sections were made ,and the histopathological changes were observed under light microscope.The expression levels of NF-κB p65,IκB-αand PIκB-αin lung tissues were detected by Western blotting .Results Luteolin could obviously inhibit Ri ,Re and reduce Cdyn ,and could significantly decrease the concentrations of IL-1β,IL-6 and TNF-αin BALF ( P <0.05),as a result,which could relieve lung injury ,moreover,luteolin could inhibit the activation of NF-κB p65 and PIκB-α.Conclusion Luteolin has a protective effect on ALI induced by LPS in mice , and its action mechanism is correlated to inhibiting the expression of NF-κB signal transduction pathway .
Keywords：luteolinacute lung injurylipopolysaccharidepulmonary function
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 339-342 )