Correlation between serum levels of MIF as well as 173G/C polymorphism and pathogenesis of psoriasis in Tujia populationof Enshi District

ZHOU Faqiong; LEI Shuying; WU Mingzhu

doi:10.3969/j.issn.1002-7386.2015.03.002

Abstract：Objective To investigate the levels of macrophage migration inhibitory factor ( MIF) in serum of patients with psoriasis in Tujia population of Enshi District , and to explore the correlation between the pathogenesis of psoriasis and 173 G/C polymorphism .Methods A total of 245 patients with psoriasis ( case group ) who were admitted to our hospital from September 2009 to September 2010 and 260 healthy volunteers of Tujia population in the same period were enrolled in the study.The serum levels of MIF were detected by ELISA , and MIF-173 G/C was genetyped by SNaPshot SNP technique . Finally,MIF-173 G/C genotype and serum levels of MIF were analyzed and compared between the two groups .Results The serum levels of MIF in case group were significantly higher than those in control group [(804.21 ±69.78)pg/ml vs (369.46 ±60.46)pg/ml, P <0.01].There were significant differences in the 173G/C genotype of MIF gene and allele frequency between two groups ( P <0.05).The CC genotype and C allele frequency in case group were significantly higher than those in control group ( P <0.01).As compared with GG genotype,the relative risk exposed to CG,CC genetype was 1.46 (95%CI:1.01~2.10, P <0.05),5.19 ( 95%CI:1.69~15.89, P <0.01),respectively.The clinical characteristics were compared in the patients with three different kinds of genotype (GG,GC,CC) of MIF173G/C polymorphism,which showed that there was a significant difference in family history ,psoriasis vulgaris ( P <0 .05 ) .Conclusion MIF-173 G/C polymorphism may be one of genetic susceptibility factors in pathogenesis of psoriasis in Tujia population of Enshi District , and its potential mechanism is that the polymorphism may affect the expression levels of MIF .