Expression and clinical significance of Slug and SRF in esophageal squamous cell carcinoma

HE Xi; LI Wei; WANG Zhiqiang

doi:10.3969/j.issn.1002-7386.2015.02.008

Abstract：Objective To investigate the expression and clinical significance of zinc finger transcription factor-Slug and serum response factor ( SRF ) in esophageal squamous cell carcinoma ( ESCC ) .Methods One hundred and twenty specimens from ESCC radical surgery without preoperative chemotherapy and radiotherapy were collected from September 2010 to September 2013 .All of ESCC tissues ( ESCC group ) were embedded by paraffin after operation .The other 60 cases of gastric tissues ( control group ) that were 5 cm away from ESCC tissue were served as normal control group .The expression levels of Slug and SRF protein were detected by immunohistochemistry (SABC method).At the same time,the correlations between the expression levels of Slug&SRF and clinical biological behavior of ESCC ,including tumor size ,differentiation degree ,depth of infiltration,lymph node metastasis were analyzed .Results The positive expression rates of Slug and SRF protein in ESCC group were 61.67% (74/120) and 46.67% (56/120),respectively,which were significantly higher than those in control group (13.13%,4/30) and (20.00%,6/30),respectively ( P <0.05).There was no significant correlation between the expression levels of Slug protein and tumor size , differentiation degree , depth of infiltration , lymph node metastasis ( P >0.05);nor was SRF protein ( P >0.05).However the expression levels of Slug and SRF protein of ESCC in serous membrane+serous membrane outside group were significantly higher than those in mucous membrane +muscular layer group ( P <0.05).Conclusion The overexpression of Slug and SRF may be related to the depth of tumor infiltration ,which may be involved in the pathogenesis and development of ESCC and may play an important role in the invasion of ESCC .