Experimental study on the effect of GLSP on human ovarian cancer cell proliferation and apoptosis in vitro
Abstract：Objective To investigate the effect of Ganoderma lucidum spore powder ( GLSP) on cell proliferation and apoptosis of human ovarian cell line-OV 2008,and to explore its action mechanism .Methods The effects of GLSP on OV2008 cell growth and cell morphous were detected by WST-1 colorimetric technique and microscopy; cell cycle was analyzed flow cytometry;cell apoptosis was detected by Hoechst-33258 staining method;the expression of apoptosis-related proteins ( bcl-2 bax,P27,cleaved Caspase-3) were measured by Western blotting .Results The results of WST-1 assays showed that GLSP could inhibit the growth of OV2008 cells,moreover,which was time and dose dependent .The results by flow cytometry showed that GLSP could block OV2008 cell growth at G1 phase.Hoechst 33258 staining results showed that GLSP could induce cell apoptosis.Western blotting results showed that the expression levels of bcl-2, bcl-xl were decreased with the increase of concentration of GLSP,however bax,P27,Caspase-3 could inhibit the growth of ovarian cancer cells and induce cell apoptosis , and its action mechanism was that the expression levels of Casbase-3 that affected cell cycle were gradually increased . Conclusion GLSP can inhibit the growth of ovarian cancer cells , block cell growth at G1 stage, furthermore, which can develop its effects by up-regulating the expression of bax , P27, down-regulating the expression of bcl-2 and activating Caspase-3.
Keywords：Ganoderma lucidum spore powderovarian neoplasmsproliferationapoptosiscell cycle
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 165-169 )