The significance of differential diagnosis of detection of TERC gene by FISH technique in paraffin section between CIN1 and CIN2

HE Chunnian; XU Mingtang; XUN Wenjuan

doi:10.3969/j.issn.1002-7386.2014.24.001

Abstract：Objective To investigate the practical significance of detection of telomerase RNA gene ( TERC ) by FISH technique in paraffin section in differential diagnosis between grade 1 cervical intraepithelial neoplasia (CIN1) and grade 2 cervical intraepithelial neoplasia (CIN2).Methods The 137 specimens of cervical tissue from surgical ablation or biopsy were enrolled in the study in which there were 20 cases of normal cervical squamous epithelium tissues ,46 cases of CIN1,49 cases CIN2 and 22 cases of CIN1/CIN2 that were difficult to be identified by HE staining .The TERC gene amplification status was detected by FISH method in paraffin section .Results The increase of TERC gene copy number was not found in normal cervical squamous epithelium .TERC gene amplification rate was 15.2%(7/46) in CIN1,47%(23/49) in CIN 2,27.8%(5/22) in CIN1/CIN2,respectively.There were significant differences between any two groups ( P <0.05).There was no significant difference in TERC gene amplification rate between normal cervical squamous epithelium and CIN 1( P >0.05), however,there was a significant difference between normal cervical squamous epithelium and CIN 2 ( P <0.05),moreover, there was a significant difference between CIN 1 and CIN2 ( P <0 .05 ) .The sensitivity of TERC gene amplification in differential diagnosis between CIN1 and CIN2 was 46.9%, specificity was 84.7%, accuracy was 65.0%, and positive predictive value was 76 .6%, negative predictive value was 60%.Conclusion The detection of TERC gene by FISH technique in paraffin section for differential diagnosis between CIN 1 and CIN2 is feasible and reliable , which has practical value and wide application prospect .