Effect of the adr enomedullin on the signaling pathwa y of PI3K/Akt/eNOS/VEGF in renal tumor cell
Abstract：Obj ective To investigate the effect of the adrenomedullin on the signaling pathway of PI3K/Akt/eNOS/VEGF in renal tumor cell.M ethods The kidney tumor cells were divided into five groups,control group,ADM group,VEGF group, ADM +VEGF receptor inhibitor group, ADM +ADM shRNA group, moreover, every group was redivided three time gradient groups (24 hours,48 hours and 72 hours).The expression levels of PI3K,Akt,eNOS, VEGF in control group and ADM group and the expression levels of P3I K,Akt, eNOS in VEGF group,ADM+VEGF receptor inhibitor group,ADM +ADM shRNA group were detected by Western Blot,respectively.Results The expressions levels of the PI3K,Akt,eNOS,VEGF in ADM groups were significantly higher than those of control group ( P <0.05) .The expression levels of PI3K,Akt,eNOS in VEGF groups and ADM+VEGF receptor inhibitor groups were significantly higher than those of control group ( P <0.05). However there were no significant differences in the expression levels of PI3K, Akt, eNOS between ADM +ADM shRNA groups and control group ( P <0.05).C onclusion ADM can activate PI3K/Akt/eNOS signaling pathway in renal tumor cell,which can not be blocked by VEGF receptor inhibitor.On one hand ADM regulates renal tumor angiogenesis by VEGF,on the other hand,by activating PI 3K/Akt/eNOS signaling pathway to participate in renal tumor angiogenesis.ADM may become a promising anti-angiogenic target in the treatment of renal tumor.
Keywords：adrenomedullinvascular endothelial growth factorphosphatidylinositol 3-kinaseprotein kinase Bendothelial nitric oxide synthaserenal tumor
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3525-3527 )