Study of exogenous AFP on hepatoma cells proliferation and anti-apoptotic effect
Abstract：Objective To investigate the biological functions and significance of exogenous AFP in hepatoma cell lines.Methods Two types of normal hepatoma cell lines ( HepG2 and HLE) were chosen,added with different concentrations of exogenous AFP for 24 hours,MTT was used to detect cells proliferation;ATRA and AFP were employed to stimulate cells at the same time,FCM was used to detect cells apoptosis.Results For the HepG2 and HLE cells with exogenous AFP, MTT results showed that AFP could increase cell proliferation significantly.Comparing with control group,when cells were treated with ATRA alone,cells apoptosis were significantly increased,while after adding exogenous AFP,cell apoptosis were partially reversed.Conclusion Exogenous AFP can not only promote the proliferation of hepatoma cell lines,but also enhance the cells anti-apoptosis ability.
Keywords：alpha-fetoproteinhepatic carcinomacell proliferationapoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3369-3371 )