I nhibitory effect of end co annabinoid anandamideo n L-type calcium current by regulating protein kinase C activity
Abstract：Objective To investigate whether anandamide, one of endocannabinoids, can inhibit L-type calcium current by rebulating protein kinase C ( PKC ) activity, and to explore the possible signal pathway that may change PKC activity.Methods L-type calcium current of single cardiomyocyte was recorded by whole cell patch clamp technique;the PKC activity was detected by PepTag non radioactive protein kinase C detection system ( Promega);DAG content within cell was determined by ELISA kit;the expressions of PLCβand p-PLCβwere detected by Western Blot.Results Anandamide significantly inhibited L-type calcium current after perfusion of myocytes ( P <0.05),and the effect could be blocked by pretreatment of CB1 receptor antagonist AM251 or PKC nonspecific agonist PMA,however,CB2 recepor antagonist AM630 had no the effect.Anandamide could inhibit significantly PKC activity ( P <0.05),similarly,pretreatment of AM251 or PMA could block the effect,however,AM630 had no the effect.Anandamide had no effect on DAG content as well as expressions of PLCβand p-PLCβ.Conclusion The experimental results demonstrate that endocannabinoid-anandamide can inhibit PKC activity by activating CB1 receptor to inhibit L-type calcium current,however,PLCβ-DAG signal pathway is not involved in the process.
Keywords：endocannabinoidendocannabinoid receptoranandamideprotein kinase CL-type calcium channel
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 3365-3369 )