The establishment of screening model of L-ornithine decarboxylase inhibitor
Abstract：Objective To evaluate the effect of screening drugs on activity of L-ornithine decarboxylase ( L-ODC) in PC-3M cells by means of HPLC,and to establish the screening model of L-ornithine decarboxylase inhibitor.Methods A TSKge lODS-80TM column (150mm ×4.6mm,5μm) was used for chromatographic separations,in gradient conditions at 0.8ml/min,with the mobile phase composed by water-methanol.The initial mobile phase was composed by 80% methanol, 20%water,maintained for 3min.Then during 4~14min,the composition was linear changed to 100%methanol,maintained for 3 min.The excitation wavelength was set at 340nm and emission wavelength was 515nm.The injection volume was 10μl. Results The inhibition rate of difluoromethylornithine (DMFO) to the activity of L-ODC was(59.3 ±5.4)%,the inhibition rate of rapamycin to activity of L-ODC was (29.8 ±6.4)%and the inhibition rate of curcumin to the activity of L-ODC was (26.1 ±6.6)%.Conclusion The model established by detecting the effect of L-ornithine decarboxylase in cells and detecting the activity of L-ornithine decarboxylase by means of HPLC may become a rapid, accurate, convenient detection method for screening ODC inhibitor.
Keywords：L-ornithine decarboxylaseL-ornithine decarboxylase inhibitorscreening model
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3217-3219 )