Effect of EGCG’ s in enhancing chemotherapy sensitivity of 5-fluo rouracil on human esophageal cancer line C aEs-17 in vitro

doi:10.3969/j.issn.1002-7386.2014.21.002

Abstract：Objective To observe the effect of epigallocatechin-3-gallate ( EGCG ) in enhancing chemotherapy sensitivity of 5-fluorouracil ( 5-Fu ) on human esophageal cancer line-CaEs-17 in vitro, and to explore possible action mechanism.Methods The experiment consisted of four groups:blank control group,5-Fu group ( final concentration of 5-Fu:6μmol/L in culture medium),EGCG group (final concentration of EGCG:20mg/L),combination group (final concentration of EGCG:20mg/L and 5-Fu:6μmol/L),affected for 24 hours in four groups.The inhibitory rate on cell proliferation and survival rate were detected by CCK-8 method and cell clone formation test,respevtively;the synergism effect of two drugs was evaluated according to Jin’ s formula;the cell apoptosis rate and cell cycle distribution were detected by flow cytometry;cell invasiveness was determined by Transwell method;the expression levels of mutant P53 (mtP53) protein were detected by Western Blot. Results Both EGCG and 5-Fu could inhibit the proliferation of CaEs-17 cells,induce cell apoptosis ,decrease cell survival rate and cell invasiveness, moreover, both EGCG and 5-Fu could block cell at G0/G1 stage and cloud down-regulate the expression levels of mtP53 protein ( P <0.05),the effects of combined application of two drugs were more obvious ( P <0.01).Conclusion EGCG can enhance chemotherapy sensitivity of 5-Fu on human esophageal cancer line-CaEs-17 in vitro, which is performed partly through down-regulating the expression levels of mutation P53 protein.