Effects of three kinds of IL-15 gene transfection on apoptosis of NCI-H446 cell
Abstract：Objective To investigate the effect of three kinds of IL-15 gene transfection on apoptosis of NCI-H446 cell.Methods The three kinds of NCI-H446 cell models were transfected respectively by IL-15 prototype gene,IL-15 mature peptide gene,IL-15 modified gene (CIL-2sp-IL-15mp) in which the signal peptide of IL-15 was replaced with that of IL-2,and the three different kinds of IL-15 gene transfection models were regarded as experimental groups ,besides,the wide NCI-H446 cells ( Cw) were used as control group .The cell apoptosis rate and expression levels of Fas protein were detected by flow cytometry.Results As compared with those in control group ,the cell apoptosis rate and expression levels of Fas protein were increased in CIL-15,CIL-15mp,CIL-2sp-IL-15mp in turn ( P <0.05 or P <0.01).Conclusion The three kinds of IL-15 genes transfection can result in obvious increase of apoptosis rate of NCI -H446 cell,and the increase intensity is in turn IL-15 modified gene >mature peptide gene>prototype gene ,which may be correlated with the increase of the expression levels of Fas protein.
Keywords：IL-15gene transfectionlung cancer cellsapoptosisFas
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 3049-3050 )