Study on the preparation and stability of flexible nano-liposome loaded with nerve growth factor
Abstract：Objective To study suitable preparation condition and stability of flexible nano-liposome ( FNL) loaded with nerve growth Factor ( NGF ) .Methods The FNL loaded with NGF was prepared by the method of filming and ultrasounding .The levels of NGF were detected by ELISA .The grain size of FNL was measured by laser grainsize analyzer . Results The optimal temperature of preparing FNL loaded with NGF was 35℃.The envelopment rate of prepared FNL peaked in pH 7.0.The suitable concentration of phosphatide was 40mg/ml.The best ratio of cholesterin:phosphatide was 1∶4.There was no obvious change in the grain size of FNL loaded with NGF after it was conserved in ambient temperature for 30 days and at 4℃in refrigerator for 12 months.Conclusion The study determines initially suitable preparation condition for FNL loaded with NGF,and prepared FNL has better stability .
Keywords：nerve growth factorflexible nano-liposomepreparation conditionstability
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2898-2899 )