Effects of atomization inhalation of low-dose dexmedetomidine on inflammatory response of one-lung ventilation in rats

ZHU Xiaobing; LIU Zhiqun; WU Lun

doi:10.3969/j.issn.1002-7386.2014.18.007

Abstract：Objective To investigate the effects of atomization inhalation of low-dose dexmedetomidine on inflammatory response of one-lung ventilation ( OLV) in rats. Methods Thirty-six healthy male Wistar rats weighed 300~350g were randomly divided into 3 groups,with 12 rats in each group:control group,group I and group V.The rats in group I received dexmedetomidine 1μg /kg iv over 10min, however, the rats in group V inhaled aerosolized dexmedetomidine 0.1μg/kg before one-lung ventilation.The arterial blood specimens were collected before dexmedetomidine administration (T0,baesline),at 120min after OLV (T1) and at 30min after reviving two-lung ventilation (T2) for detecting plasma concentrations of TNF-α,IL-6 and IL-10 by ELISA.The animals were then executed and their lungs were taken out to be observed under microscope,and E/D weight ratio in left lung was measured.Results As compared with those in T0,the plasma levels of TNF-α,IL-6,IL-10 in T1 and T2 of the three groups were significantly increased ( P <0.05).As compared with those in control group ,the the plasma levels of TNF-α, IL-6 and W/D ratio in T1 and T2 were significantly decreased , however,the levels of IL-10 were obviously increased in group V and group I ( P <0.05).There were no significant differences in the levels of TNF-α,IL-6,IL-10 of plasma and lung tissue in group V and group I in different time points ( P >0.05).Moreover the pathological injury of lung tissue in group V and group I was obviously relieved ,as compared with that in control group .Conclusion The atomization inhalation of low-dose dexmedetomidine can inhibit the inflammatory response caused by one-lung ventilation in rats ,and the effect is similar to that of intravenous injection of high-dose dexmedetomidine .