The study on the expression of TNF-αand Fas/FasL in placenta of patients with pre-eclampsia

doi:10.3969/j.issn.1002-7386.2014.17.004

Abstract：Objective To observe the expression of TNF-αand Fas/FasL in placenta of patients with pre-eclampsia and normal late pregnancy women ,and to explore related cause of disease and pathogenesis of pre -eclampsia.Methods Eighty puerperas who underwent cesarean section including 28 cases mild pre-eclampsia (mild pre-eclampsia group),22 cases of severe pre-eclampsia (severe pre-eclampsia group ),30 cases of normal pregnancy (control group ) were enrolled in the study.The gestational weeks,neonate’ s weight and placental weight were detected for three groups .The placenta specimens within 30min after placental expulsion were collected and fixed in 10% neutral formaldehyde for 24h for serial section,then stained with HE .The expression of TNF-α and Fas/FasL in placenta specimens was detected by immunohistochemistry .Results There were no significant differences in the gestational weeks among mild pre -eclampsia group,severe pre-eclampsia group,control group ( P >0.05).The neonate’s weight and placental weight were decreased with the aggravation of pathogeneticcondition of pre-eclampsia ( P <0.05).The expression levels of TNF-α in placenta tissue of patients with pre-eclampsia weresignificantly higher than those of control group ( P <0.05).The expression levels of Fas in placenta trophocyte and deciduasbasalis cells of patients with mild and severe pre -eclampsia were significantly higher than those in control group ( P <0.05).The coloring of FasL in placenta trophocyte and decidua basalis cells of patients with mild and severe pre -eclampsia wasattenuated,as compared with that in control group ( P <0.01),moreover,the expression levels of FasL were further decreasedwith the aggravation of pathogenetic condition of pre -eclampsia.Conclusion The abnormal expression of TNF-α and Fas/FasLin placenta may play an important role in the pathogenesis of pre -eclampsia.