Effect of Jinlida on the expression of GLUT4 in skeletal muscle of rats with insulin resistance

doi:10.3969/j.issn.1002-7386.2014.15.006

Abstract：Objective To investigate the effect of Jinlida on the expression of GLUT 4 in skeletal muscle of rats with insulin resistance .Methods Sprague Dawley ( SD ) rats were randomly divided into four groups:normal diet group ( ND group),high-fat diet group (HFD group),high fat diet with Jinlida group (Jinlida group) and high fat diet with pioglitazone group (pioglitazone group),with 6 rats in each group.After 8 weeks,intraperitoneal glucose tolerance test (IPGTT) was performed and area under the curve ( AUC) was calculated based on the blood glucose curves;blood glucose and insulin were detected to calculate HOMA-IR;the expression levels of GLUT 4 in skeletal muscle were detected by quantitative real-time polymerase chain reaction (RT-PCR) and Western Blot.Results Jinlida administration obviously improved 30min,60min blood glucose and AUC during IPGTT,pioglitazone improved significantly 60min,120min blood glucose and AUC ( P <0.05),both Jinlida and pioglitazone improved obviously HOMA-IR index ( P <0.05),and both of them up-regulated the expression of GLUT4 in skeletal muscle ( P <0.05).Conclusion Jinlida can up-regulate expression of GLUT4 in skeletal muscle of rats,as a result,which can improve insulin resistance induced by high fat diet .