Expression and significance of double negative T cells and lymphocyte subtype in patients with chronic severe hepatitis B
Abstract：Objective To investigate the expression and significance of CD 3+CD4-CD8-( DNT) cells and lymphocyte subtype in patients with chronic severe hepatitis B .Methods The serum samples from 32 healthy subjects ,40 patients with chronic hepatitis(CHB)and 35 patients with chronic severe hepatitis B (CSHB) were collected in this study.The DNT cells and lymphocyte subtype in peripheral blood were detected by flow cytometry .Results The percent of DNT cells in CHB and CSHB groups [(8.44 ±4.25)%and (10.78 ±5.33)%,respectively] were significantly higher than those in control group [(6.23 ±3.77)%, P <0.05],moreover,the increase extent in CSHB group was more obvious than that in CHB group ( P<0.05).The HBV DNA load was positively correlated to DNT cells ( r =0.742, P <0.05).The CD4++T lymphocytes in CHB group were significantly decreased ,as compared with those in control group ( P <0.05 ) .The percentages of CD 4+T lymphocytes and the ratio of CD4+/CD8+ in CSHB group were significantly decreased , however, CD8+ T lymphocytes were significantly increased,as compared with those in CHB group ( P <0.05).Conclusion The obvious increase of DNT cells and the abnormality of T lymphocyte subset percentage in patients with chronic severe hepatitis B may be one of important reasons for the pathogenesis and development of chronic hepatitis B .
Keywords：chronic severe hepatitis Bdouble negative T cellsT-lymphocytes subsets
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2248-2249,2250 )